Increased CD4⁺ T cell apoptosis in <i>pik3cg^−/−</i>

<p><b>mice during EAE.</b> (<b>A</b>) The proportion of apoptotic (Annexin V⁺/Propidium iodide⁺) cells in the spinal cord was higher in <i>pik3cg^−/−</i> mice compared with WT. Representative dot plots gating on CD4⁺ lymphocytes harvested from spinal cord taken from mice on day 9 and day 15 of EAE are shown. (<b>B</b>) Increased activation induced cell death in <i>pik3cg^−/−</i> CD4⁺ T cells. Splenocytes were harvested from naïve WT and <i>pik3cg^−/−</i> mice and cultured on anti-CD3 coated tissue culture trays for 4 days in the presence of anti-CD28 antibodies. Every 24 hours from day 1 onward, cells were collected and analysed for the presence of apoptotic (Annexin V⁺/Propidium iodide⁺) CD4⁺ T cells by flow cytometry. In all cases 5–8 mice were included in each group. Data are mean ± s.e.m.