Tregs preserve dendritic cell transcription of IL-2.

<p><b>A:</b> DO11.10 Tregs and C57BL/6 splenic DCs were cultured together or in separate tubes for 5 and 24 h, IL-2 message was measured in cultured cells by RT-qPCR as in materials and methods. Bars are the mean ± SE of IL-2 message in the cultures. Statistical analysis was by one-way repeated measures ANOVA and Tukey's test for all pairwise multiple comparison procedure. <b>B&C:</b> BMDCs were transfected with the IL-2 reporter plasmid (pIL2P8.4-EGFP), a negative control plasmid (pcDNA3.1/V5-His B), and a positive control plasmid to assess transfection efficiency (pAcGFP-N1). Transfectants were analyzed for IL-2-promoter activity in cells as in materials and methods. <b>B:</b> Panels show histograms of the level of promoter-driven GFP in gated CD11c⁺ cells. The experiment was repeated with similar results. <b>C:</b> pIL-2P8.4-EGFP-transfected BMDCs were cultured unstimulated or with CpG-B, with and without Treg cells for 40 h. Bars are the mean ± SE of IL-2-promter-EGFP⁺ BMDCs from two experiments.