The region between F343 and Y337 is vital for the Hog1^D170A protein.

<p><b>A.. </b><i>hog1</i>Δ cells harboring vectors expressing either Hog1^WT, Hog1^D170A, Hog1^{(Y337–F343)Δ}, or Hog1^{(Y337–343)Δ+D170A} were plated in five dilutions on plates containing YPD supplemented with 1 M NaCl (left panel). All strains were also plated on medium with no NaCl and all grew equally well on these plates (not shown). Phosphorylation levels of Hog1^WT, Hog1^D170A, Hog1^{(Y337–F343)Δ}, or Hog1^{(Y337–343)Δ+D170A} expressed in <i>hog1</i>Δ cells were tested in cells exposed or not exposed to NaCl (right panel). <b>B.. </b><i>hog1</i>Δ<i>pbs2</i>Δ cells harboring either an empty vector, or vectors expressing either Hog1^WT, Hog1^D170A, Hog1^{(Y337–F343)Δ}, or Hog1^{(Y337–343)Δ+D170A} were plated in five dilutions on plates containing YPD supplemented with 1 M NaCl (left panel). All strains were also plated on medium with no NaCl and all grew equally well on these plates (not shown). Phosphorylation levels of Hog1^WT, Hog1^D170A, Hog1^{(Y337–F343)Δ}, or Hog1^{(Y337–343)Δ+D170A} expressed in <i>hog1</i>Δ<i>pbs2</i>Δ cells were tested in cells exposed or not exposed to NaCl (right panel).