The effects on cell proliferation and cell size.

<p>(A–B) the effects of location-specific activation of EGFR on cell proliferation. CHO-EGFR and CHO-LL/AA cells were stimulated with EGF (50 ng/ml) for the indicated times. the cell proliferation was measured by the fold of increase of the cell numbers (A) or by the BrdU incorporation (B). (C–D) The effects of location-specific EGFR activation on cell size. For C, the cell sizes of CHO-EGFR and CHO-LL/AA cells were measured and compared. For D, the 293T cells were transiently transfected with either the YFP-tagged wild type EGFR or mutant EGFR1010LL/AA. Three days later, the cell sizes were measured and compared. The cell sizes were determined by the diameter of the cells. The calculation of the diameter was described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041354#s2" target="_blank">Materials and Methods</a>. * indicates that the difference is significant (P<0.05). ** indicates that the difference is very significant (P<0.01).