Proliferation of tumor cells in three-dimensional type I collagen gel in the presence of FH535.

<p>Cells (HCC38, MDA-MB-231, T47D, Sk-Br3, MCF-7) were cultured in type I collagen gel as described in material and methods. Cell/gel matrices were fixed and embedded in paraffin. The paraffin section was serially cut at 5 m and mounted on slide glasses. Tissue sections were stained with anti-Ki67 antibody or control IgG followed by HRP-conjugated secondary antibody as visualizing by DAB staining with counter staining by DAPI to localize cells. The results were demonstrated by the (mean +/− standard deviation) of % of positive cells for DAB from three independent experiments. Insets showed the typical staining pattern of anti-Ki67 antibody in HCC38 and MDA-MB-231 cells in the presence or absence of FH535 at a concentration of 10 µM. Magnification x200. * <i>p</i><0.001 (by Student’s two-tailed paired <i>t</i>-test). n.s: not significance (by Student’s two-tailed paired <i>t</i>-test).