Effect of blockade of clathrin-mediated endocytosis on DENV-1 and DENV-2 strains.

<p>A. Cells were treated with 50 µM chlorpromazine and then infected with reference strains and clinical isolates of DENV-1 or DENV-2. Virus yields were quantified by PFU at 48 h p.i. and results are expressed as % of inhibition of virus multiplication with respect to a control of infected cells without drug treatment. Each value is the mean±SD of three independent experiments. B. Cells were treated with 50 µM chlorpromazine or left untreated and incubated with TRITC-labelled transferrin. C. Cells transiently transfected with the constructs GFP-DIIIΔ2 or GFP-EH29 were infected with DENV-2 strain 16681. After 24 h cells were fixed and viral antigen expression was visualized by immunofluorescence staining using mouse anti-E glycoprotein antibody and TRITC-labelled anti-mouse IgG.