Effect of Notch1 silencing on D, L-sulforaphane (SFN)-mediated inhibition of PC-3 and LNCaP cell migration.

<p>(A) Immunoblotting for full-length Notch1 protein using lysates from PC-3 and LNCaP cells transiently transfected with a control (nonspecific) siRNA or a Notch1-targeted siRNA. (B) Representative images (Boyden chamber assay) depicting migration of PC-3 and LNCaP cells transfected with a control (nonspecific) siRNA or a Notch1-targeted siRNA and treated for 24 hours with DMSO or 10 μM SFN (×100 magnification). (C) Quantitation of PC-3 and LNCaP cell migration from data shown in panel B. Two to three fields on each filter were scored for cell migration under an inverted microscope. Data represent percent cell migration normalized to control siRNA-transfected cells treated with DMSO (mean ± SD, n = 6; combined data from two independent experiments each performed in triplicate). Significantly different (<i>P</i><0.05) ^acompared with respective DMSO-treated controls (cells transfected with control siRNA or Notch1-targeted siRNA), and ^bbetween control siRNA transfected cells and Notch1-targeted siRNA transfected cells by one-way ANOVA followed by Bonferroni's multiple comparison test. Each experiment was performed twice.