Expression levels of Ang4 in the colons of mice after <i>T. muris</i> infection.

<p>A; Ang4 mRNA expression was assessed by Q-PCR in samples of colonic tissue from AKR (n = 4–5), BALB/c (n = 4–5) and C57BL/6 mice (n = 4–5) at various times post-infection. B; Quantification of Ang4 protein expression by IHC in colonic tissue from AKR, BALB/c and C57BL/6 mice at various times post-infection. C; examples of immunohistochemical staining for Ang4 in samples of colonic tissue from AKR, BALB/c and C57BL/6 mice at various times post-infection. Original magnification ×200. Arrowheads indicate <i>T. muris</i> worm cross-sections in tissue. D; Ang4 mRNA expression was assessed by Q-PCR in samples of colonic tissue from SCID mice (n = 4) at various times post-infection. E; Quantification of Ang4 protein expression by IHC in colonic tissue from SCID mice at various times post-infection. Changes in Ang4 protein levels were confirmed in a second independent experiment in naïve mice and at day 21 post-infection.*p<0.05, **p<0.01, ***p<0.001 One-way ANOVA with post-hoc Dunnett's compared to naïve or d3 control as appropriate.