Transport of DENV particles to recycling endosomes.

<p>Cells transiently transfected with the GFP-tagged versions of Rab22 wt and Q64L (A), Rab11 wt and S25N (B) and the plasmid pGFP-C1 were infected with DENV-1 HW, DENV-2 NGC or 16681. After 24 h of infection cultures were fixed and processed to visualize GFP transgene expression and viral antigen by immunofluorescence staining using mouse anti-E glycoprotein antibody and TRITC-labelled anti-mouse IgG. C. For quantification of samples shown in A and B, 250 transfected cells with similar levels of GFP expression were screened and cells positive for viral antigen were scored. D. Cells expressing GFP-Rab7 wt or Green Lantern-Rab11 wt were infected at 24 h post-transfection with DENV-2 NGC or 16681 during 60 min at 4°C and then shifted at 37°C. After 15 min cells were fixed and processed to visualize GFP transgene expression and viral antigen by immunofluorescence staining using mouse anti-C glycoprotein antibody and TRITC-labelled anti-mouse IgG. Cells were visualized with a confocal microscope.