Concentration-dependent effects of rotenone, MPP⁺, and 6-OHDA on basal OCR.

<p>Using the XF24 analyzer, the mitochondrial oxygen consumption rate (OCR) was determined for 4 basal readings with 80,000 cells plated per well. OCRs were between 8–12 pmol O₂/min/µg protein. Then rotenone (A), MPP⁺ (B), and 6-OHDA (C) were injected. (A) ▪ control, □ 1 nM rotenone, • 10 nM rotenone, and ○ 100 nM rotenone. (B) ▪ control, □ 500 nM MPP⁺, •5 µM MPP⁺, and ○ 500 µM MPP⁺. (C) ▪ control, □ 50 µM 6-OHDA, and ○ 200 µM 6-OHDA. Data are expressed as percent of the basal OCR prior to injection of neurotoxins. Data = mean ± SEM, n = 3. In some cases, the error bars are smaller than the symbols.