NF-κB is activated by phosphorylated Rac1.
- Janett Schwarz (308573)
- Julia Proff (308574)
- Anika Hävemeier (305490)
- Markus Ladwein (308575)
- Klemens Rottner (222112)
- Britta Barlag (308576)
- Andreas Pich (152212)
- Helma Tatge (308577)
- Ingo Just (308578)
- Ralf Gerhard (308579)
Publication date
February 2013
DOIAbstract
<p>HEK 293 cells were co-transfected with NF-κB luciferase reporter plasmid as well as with Rac1 and Cdc42 mutants. Cells were lysed after 40 hours and analyzed for luciferase activity. Relative fold activity of mock-transfected cells is shown (arithmetic means±SD, n = 4). B) Expression of HA-tagged GTPases of quadruplicate samples from reporter gene assays was visualized by immunoblot using an anti-HA antibody.</p
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