Mistrafficking of photoreceptor opsins in RD.

<p>Calbindin (red) and L/M opsin (green) immunostaining in control retina of the untreated fellow eye (a–c) and hyaluronic acid RD at day 3 (d–f) and 9 (g–i). Note decreased intensity of L/M opsin staining at day 9 HA RDs. Confocal microscopy demonstrates early mistrafficking of L/M opsin (green) with staining of the photoreceptor cell body and cone pedicles (arrows, k, l) in SA RDs at day 3 compared to untreated controls. The L/M opsin staining is present in areas consistent with the cone IS/OS as well as the cone ellipsoids in normal chick retina (j). Some transitin-positive glial processes are seen extending beneath the outer limiting membrane (OLM, arrowhead k, l). Similar to day 3 HA RDs, day 3 SA RDs show mistrafficking of rhodopsin (red) and L/M opsin (green, m, n) and S opsin (green, o, p), despite resolution of subretinal fluid. In advanced HA RD (Day 16 RD, q–t) there is redistribution of L/M opsin and calbindin to the cell body with loss of S opsin and rhodopsin (bracket). ONL (outer nuclear layer), OPL (outer plexiform layer), PRL (photoreceptor layer). Scale bar 50 microns.