The effect of bezafibrate on the skeletal muscle of Mut and WT mice.

<p>(A) Western blot of PGC-1α and loading control actin in total quadricep homogenate from 10 month-old mice and quantification of PGC-1α band intensity normalized to actin (n = 4/group). (B) Gene expression of <i>PGC-1β</i> and <i>PPARs</i> in the quadricep of 10 month-old mice normalized to actin (n = 4/group). (C) Citrate synthase activity in the total quadricep homogenate from 10 month-old mice (n = 4/group). (D) Quantification of western blot of mitochondrial proteins in the total homogenate from the quadricep of 10 month-old mice (n = 4/group). NADH dehydrogenase (ubiquinone) 1β subcomplex subunit 8 (NDUFB8; subunit of complex I), succinate dehydrogenase subunit B (SDHB; subunits of complex II), ubiquinol-cytochrome <i>c</i> reductase core protein 2 (UQCRC2; subunit of complex III), mitochondrial cytochrome c oxidase subunit 1 (MTCO1; subunit of complex IV) and ATP synthase subunit 5α (ATP5A; subunit of complex V) (E) Quantification of mtDNA levels in the quadriceps of 10 month-old mice based on ND1 (subunit of complex I) levels normalized to glyceraldehyde 3-phosphate (GAPDH) (n = 4/group). (F) Gene expression of markers of fatty acid oxidation, acyl-coenzymeA oxidase 1 (ACOX), cluster of differentiation 36 (CD36), carnitine palmitoyl transferase (CPT1) and short-chain-acyl-coenzymeA dehydrogenase (SCAD) in the quadriceps of 10 month-old mice normalized to actin (n = 4/group) (G) Skeletal muscle weight of 10 month-old mice (n = 4–6/group). (H) The number of falls of mice when put to run on a treadmill for 3 minutes at 9 meters/minute (n = 5–10/group). *, <i>P</i><0.05; **, <i>P</i><0.01, ***<i>P</i><0.001, Student’s <i>t</i>-test. Error bars represent the SEM.