<i>RBMS3</i>-silenced NP460 cell showed malignant features.

<p>(<b>A</b>) siRNA against <i>RBMS3</i> effectively reduced the mRNA and protein expression of RBMS3 in NP460 cells compared to siScramble-transfected cells. (<b>B</b>) An MTT assay was used to compare cell growth rates between siRBMS3- and siScramble-transfected NP460 cells (* p<0.05; ** p<0.001, Student’s <i>t</i>-test). Values were expressed as mean ± SD of three independent experiments. (<b>C</b>) Foci formation assay showed that the number of foci was significantly increased in siRBMS3-transfected NP460 cells compared to control cells (* p<0.05, Student’s <i>t</i>-test). The results were expressed as mean ± SD of three independent experiments. (<b>D</b>) Representative and summary of DNA content detected by flow cytometry showed that the percentage of cells in the S phase was higher while the percentage of cells in the G1 phase was lower in siRBMS3-transfected NP460 cells than that in control cells. (* p<0.05, Student’s <i>t</i>-test). (<b>E</b>) Expression of <i>VEGF</i> in siRBMS3- and siScramble-transfected NP460 cells was confirmed by qPCR. (* p<0.05, Student’s <i>t</i>-test). The results were expressed as mean ± SD of three independent experiments. (<b>F</b>) β-catenin was significantly up-regulated while p53 was significantly down-regulated in siRBMS3-transfected NP460 cells compared to control cells by Western blot analysis. β-actin was used as a loading control.