<i>cblb</i> silencing substantially enhances tumor infiltration of T cells.

<p>Flow cytometric analysis of tumor-infiltrating lymphocytes 7 days (A, B, D, E) or 4 days (C) after a single ACT in combination with DC vaccination into wild type mice was performed. (A) CD8⁺ T cell infiltrates gated on whole tumor, (B) CD8⁺ T cell infiltrates gated on total CD45⁺ leukocytes, (C) SIINFEKL-specific infiltrates gated on CD8⁺ cells (D) CD4⁺CD25^- T cell infiltrates and (E) CD4⁺CD25⁺ T cell infiltrates gated on total CD45⁺ leukocytes are shown. Means ± SEM of two independent experiments are shown (n = 7 - 8 mice per group). (A) p = 0.06 (DC+CD8⁺<i>cblb</i> siRNA versus DC+CD8⁺ control siRNA) (B-E) n.s. (DC+CD8⁺<i>cblb</i> siRNA versus DC+CD8⁺ control siRNA).