Resveratrol activates the PI3-K/Akt signaling pathway in RAW 264.7 macrophage cells.

<p>Panel A shows that RAW 264.7 cells were treated with resveratrol (5 µM) for the indicated times, Panel B shows that RAW 264.7 cells were pre-treated with resveratrol (5 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 40 min and Panel C shows that the cells were incubated with LPS (5 µg/ml) without or with 5 µM resveratrol for the indicated times. Various treated RAW 264.7 cell lysates (50 µg protein) were prepared and subjected to Western blot analysis by using antibodies specific for total Akt and phosphorylated forms of Akt (shown as phospho-Akt) as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044107#s4" target="_blank">methods</a>. The relative protein levels were quantified by scanning densitometry and normalized to total Akt. The values shown are mean ± SEM of data from three independent experiments. ^#Significant compared with control alone, <i>p</i><0.05. ^*Significant compared with LPS alone, <i>p</i><0.05. ^ΔSignificant compared with resveratrol + LPS, <i>p</i><0.05.