Inhibition of HMGB1 activity protects mutant mice against the lethal effects of heatstroke.

<p>(<b>A</b>) SDS-PAGE analysis of the recombinant A-Box and GST control proteins expressed and purified from <i>E. coli</i> is shown. Increasing amounts of purified proteins were resolved by SDS-PAGE and stained with Coomassie blue; (<b>B</b>) Mutant mice were pretreated (i.p. injection) with A Box 600 µg/mouse; or GST control and heatstroke was induced by passive exposure to environmental heat until the core temperature reached 42.7°C. Pretreatment with A Box before heat stress protected the mutant mice against the lethal effects of heatstroke. *P = 0.04 Kaplan-Meier log-rank testing between the two groups.