The pH value of nutrient solution in which maize plants were cultured.

<p>Maize seedlings were cultured in SP (1,000 µMKH₂PO₄) nutrient solution for 20 days, then divided into two groups and grown separately in SP and LP (5 µM KH₂PO₄) nutrient solutions for an additional 25 days. After 10 days of culturing in different Pi conditions, the pH value of nutrient solution was measured every day. Each of maize plants was cultured in 1 liter of nutrient solution. The initial pH value of nutrient solution was 6.0±0.1. Nutrient solution was replaced every 3 days. In this figure, on the 10th, 13th, 16th, 19th, 22th and 25th day of low Pi stress, the nutrient solution was replaced. Values are means ± SD (<i>n</i> = 5). WT, wild type; L1, L2 and L3, transgenic lines; SP, sufficient phosphate (nutrient solution containing 1,000 µM KH₂PO₄); LP, low phosphate (nutrient solution containing 5 µM KH₂PO₄).