PI3-K is involved in resveratrol-attenuated the production of the proinflammatory cytokine IL-1β at the transcriptional and translational levels in RAW 264.7 macrophage cells.

<p>Panel A shows the immunofluorenscence images for protein expression of IL-1β and Panel B shows the corresponding mRNA data. The relative mRNA level was quantified by scanning densitometry and normalized to β-actin mRNA. Note the up-regulated protein and mRNA expression of IL-1β by LPS is suppressed by different concentrations of resveratrol; however, in cells pretreated with PI3-K inhibitor wortmannin, the suppressive effect of resveratrol is abrogated. The values shown are mean ± SEM of data from three independent experiments. ^#Significant compared with control alone, <i>p</i><0.05. ^*Significant compared with LPS alone, <i>p</i><0.05. ^ΔSignificant compared with resveratrol + LPS, <i>p</i><0.05.