Involvement of NMDAR in HMGB1 promoted MEL cell differentiation.

<p>(<b>A</b>) 50 µg of solubilized membrane proteins from MEL cells were submitted to Western blot analysis. Immunoprecipitation of HMGB1 (IP) was carried out using solubilized MEL cell membrane proteins as specified in Materials and Methods. A representative experiment (of three) is shown. (<b>B</b>) HMBA-treated cells were exposed to the indicated additions (250 pM HMGB1, 1 µM C.I. 1, 1 µM MK-801, 50 µM CGS 19755). After 24 h the percentage of differentiated cells was evaluated by benzidine staining. Bars are means ± SD of four different experiments. *p<0.05, ^§ p<0.01, vs cells stimulated in the absence of the indicated inhibitor, according to <i>t</i> test.