Thin layer chromatography analysis of AHLs.
- Chien-Yi Chang (233503)
- Chong-Lek Koh (309437)
- Choon-Kook Sam (309438)
- Xin-Yue Chan (309439)
- Wai Fong Yin (309440)
- Kok Gan Chan (309442)
Publication date
February 2013
DOIAbstract
<p>AHL standards from Sigma (C12-HSL (**) and 3-oxo-C16-HSL (*)) and 10 µl of W3.1 and W2.3 extract were spotted on normal phase TLC plate. The TLC plate was chromatographed with dichloromethane and methanol as mobile phases. The locations of AHLs were revealed by overlay of <i>Agrobacterium tumefaciens</i> NTL4 pZLR4 as evident by blue pigmentation. Based on the R<i><sub>f</sub></i> values, W2.3 produced C12-HSL.</p
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