Survival rate of MIO-M1 cells after administration of H₂O₂ and GO and at varied concentrations and time points.

<p>Panels <b>A</b> and <b>B</b> represent the relative optical density (OD) values (positively related to number of live cells) of surviving MIO-M1 cells at 8 hrs (<b>A</b>) and 24 hrs (<b>B</b>) at various concentrations of H₂O₂ (in mM – horizontal axis). Panels C and D represent average values (±SEM) of surviving MIO-M1 cells at 8 hrs (<b>C</b>) and 24 hrs (<b>D</b>) at various concentrations of GO (in µg/L – horizontal axis). N = 6/group in every panel, error bars depict the standard error of the mean. Results showed that the survival ratio of the PRDX6 group was always higher than those in the GFP and control group after treatment of H₂O₂ and GO at varied concentrations for 8 and 24 hours. The concentration range of H₂O₂ treatment, where significant difference was observed between the PRDX6 group and the GFP or the blank group, was from 0.15 to 0.225 mM at 8 hours, and from 0.20 to 1.00 mM at 24 hours (p<0.05). After GO treatment, the concentration range where significant difference was observed ranged from 2.5 to 5.0 µg/L at 8 hours, and from 3.0 to 5.0 µg/L at 24 hours (p<0.05).