IGF1-treatment reduced the survival of colchicine-damaged ganglion cells.

<p>Retinas were obtained from eyes that were injected with saline (<b>a</b>) and colchicine (<b>b</b>) 10 days after treatment. Whole-mount preparations of the retina were labeled with antibodies to Brn3a to identify the nuclei of ganglion cells. Panel <b>c</b> is a histogram illustrating the mean number of Brn3a-positive ganglion cells per 70,000 µm² in central and peripheral regions of the retina. Significance (p-values) of difference between numbers of Brn3a-positive ganglion cells in control and treated retinas was determined by using a two-tailed Student’s t-test. The scale bar (50 µm) in panel <b>b</b> applies to panels <b>a</b> and <b>b</b>.