FACS and western blotting analysis of splenic CD4⁺CD25⁺Foxp3⁺ T cells.

<p>Splenic T lymphocytes were isolated from untreated recipients (n = 6), recipients treated with donor imDex (n = 6) or rapamycin (n = 6) alone, and imDex/rapamycin-cotreated (n = 6) recipients at d5 or d10 after transplantation. (A) The percentages of splenic CD4⁺CD25⁺ T cells from the four groups of recipient mice were calculated from flow cytometric analysis. The number in the upper-right quadrant of each histogram indicates the percentage of CD4⁺CD25⁺ T cells. Results were obtained from three mice per condition, and one representative of four independent experiments is shown. (B) Foxp3 versus β-actin western blotting analysis of splenic T lymphocytes from the groups of recipients upper. Densitometry shows fold induction of Foxp3:β-actin ratio. Bars = mean ± SD (n = 3), * <i>P</i><0.01, ** <i>P</i><0.001. One representation of three independent experiments is shown.