The effects of THL (triphala churna) on <i>in vivo</i> matrigel angiogenesis assay.

<p>(<b>A</b>) Photographs of representative matrigel plugs show THL untreated red colored vascular endothelial growth factor (VEGF) containing plugs in comparison to the VEGF minus PBS containing controls. (<b>A</b>) In contrast, THL (triphala churna) treated VEGF containing matrigel plugs were pale. (<b>B</b>) Masson's trichrome staining (endothelial cells stain red and matrigel stains blue) and (<b>C, D</b>) CD31 immunohistochemistry of the matrigel plug sections show large numbers of endothelial cells in THL untreated VEGF containing plugs in comparison to controls (+, <i>p</i><0.05). In contrast, THL treated VEGF containing matrigel plug section has considerably low numbers of endothelial cells as detected by Masson's trichrome staining and CD31 staining (*, p<0.05). Scale bars in <b>B</b> and <b>C</b>, 50 μm. <i>n</i> = six for each experimental group.