Effect of CDF and/or anti-miR-21 on miRNA expression and miR-21-mediated luciferase activity in human PCa cells under hypoxic conditions.

<p>The panels A and B represent the data of miRNAs in the CSC-like sphere-forming cells derived from human PCa cells and luciferase activities in PCa cells, respectively. The transfection of miR-21-mediated reporter gene luciferase vector and anti-miR-21 were conducted in PC-3 cells, as described in detail under the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043726#s2" target="_blank">Methods</a> section. The luciferase activity was measured by using Promega luciferase assay system kit, following the manufacturer’s manual. The bars in the figures indicate standard deviation of n = 3.