Rapamycin regulated mRNA and protein expression of eNOS in HUVECs with thrombin.

<p>After stimulation with thrombin for 4 hours, HUVECs were treated with rapamycin at concentrations of 2, 20, 200 and 2000 ng/ml for 24 and 48 hours. eNOS was assessed by real-time PCR and western blot. Each bar represented the mean±SD (n = 6). Quantitative data for the PCR and western studies were shown graphically. A, rapamycin reduced mRNA expression of eNOS for 24 hours. B, rapamycin reduced protein expression of eNOS for 24 hours. C, rapamycin reduced mRNA expression of eNOS for 48 hours. D, rapamycin reduced protein expression of eNOS for 48 hours. There was a significant decrease in mRNA and protein expression of eNOS after treating with rapamycin for 24 and 48 hours compared to stimulation with thrombin alone. (**p<0.01 for rapamycin + thrombin vs thrombin alone).