Inhibition of starvation-induced autophagic flux by MHY1485.

<p>Western blot analysis was performed to measure autophagic flux. Ac2F cells under starvation or co-treatment of 2 µM MHY1485 for 6 h were treated 1 h before the cell harvest with lysosomotropic agents bafilomycin A1 (10 nM) and chloroquine (100 µM) (A, B). Bars represent the LC3II/LC3I ratio normalized with the LC3II/LC3I ratio from untreated samples. β-Actin blot is shown to verify the same amount of protein loaded. The blots were quantified by densitometry expressed as mean±SD (*p<0.05, ***p<0.001; n = 3).