<p>(A) U2OS cells were transfected with the indicated siRNAs and harvested 72 h later. RT-qPCR was performed with primers for p21, p27 and 57. Gene expression was normalized for beta-Actin. Error bars indicate the standard error of the mean. (B) Cells were transfected with p21-Luc reporter and the indicated siRNAs. Relative light units represent the mean value of a triplicate transfection.</p
<p>(A) Measurement of mRNA (left panel) and protein (right panel) levels of p21 and PUMA by real-tim...
<p>(a) Determination of the silencing effect of six genes: APP6, ALPL, DUSP11, PPP1CC, PTPN11 and PN...
<p>Cells were collected 24 hours post-transfection for lysis, total RNA isolation, and flow cytometr...
<p>(A) U2OS cells were transfected with the indicated siRNAs. After 72 hours cells were harvested an...
<p>(A) ChIP analysis was done 46 h upon transient expression of mycPRMT6 using primers for the trans...
<p>MCF7 cells were transfected with siRNA for Sp1 (A) or c-JUN (B). 24 hours after transfection, the...
<p>The RCC (786-O and ACHN) cells were grown to ∼60% confluence and were transfected with scrambled ...
<p>(A) Q-RT-PCR shows induction of <i>p21</i> mRNA (normalized to <i>GAPDH</i>) after <i>siAPTR</i>....
<p>(a) Western blot analysis of signalling pathways in untreated MV4-11 cells (Unt) and 24 hours pos...
<p>Transcript alteration of three selected genes in PAM cells from the PRRSV-infected group compared...
<p>Protein lysates were recovered from both MRC5 and U2OS cells that were either untransfected, tran...
<p>Real-time PCR was used to measure RPS29 and CYP6N3 expression following transfection with express...
<p>(A) Transfected C6/36 cells expressing varying amounts of RPS29 (empty vector was used as a contr...
<p>(A) U2OS cells were transfected with the indicated siRNAs and fixed 72 h later. For DNA content a...
<p>(A, B) HeLa cells were transfected with varying concentrations of U2 AMO (A) or U1 AMO (B) as ind...
<p>(A) Measurement of mRNA (left panel) and protein (right panel) levels of p21 and PUMA by real-tim...
<p>(a) Determination of the silencing effect of six genes: APP6, ALPL, DUSP11, PPP1CC, PTPN11 and PN...
<p>Cells were collected 24 hours post-transfection for lysis, total RNA isolation, and flow cytometr...
<p>(A) U2OS cells were transfected with the indicated siRNAs. After 72 hours cells were harvested an...
<p>(A) ChIP analysis was done 46 h upon transient expression of mycPRMT6 using primers for the trans...
<p>MCF7 cells were transfected with siRNA for Sp1 (A) or c-JUN (B). 24 hours after transfection, the...
<p>The RCC (786-O and ACHN) cells were grown to ∼60% confluence and were transfected with scrambled ...
<p>(A) Q-RT-PCR shows induction of <i>p21</i> mRNA (normalized to <i>GAPDH</i>) after <i>siAPTR</i>....
<p>(a) Western blot analysis of signalling pathways in untreated MV4-11 cells (Unt) and 24 hours pos...
<p>Transcript alteration of three selected genes in PAM cells from the PRRSV-infected group compared...
<p>Protein lysates were recovered from both MRC5 and U2OS cells that were either untransfected, tran...
<p>Real-time PCR was used to measure RPS29 and CYP6N3 expression following transfection with express...
<p>(A) Transfected C6/36 cells expressing varying amounts of RPS29 (empty vector was used as a contr...
<p>(A) U2OS cells were transfected with the indicated siRNAs and fixed 72 h later. For DNA content a...
<p>(A, B) HeLa cells were transfected with varying concentrations of U2 AMO (A) or U1 AMO (B) as ind...
<p>(A) Measurement of mRNA (left panel) and protein (right panel) levels of p21 and PUMA by real-tim...
<p>(a) Determination of the silencing effect of six genes: APP6, ALPL, DUSP11, PPP1CC, PTPN11 and PN...
<p>Cells were collected 24 hours post-transfection for lysis, total RNA isolation, and flow cytometr...