Knockdown of PRMT6 results in an accumulation of cells in G2.

<p>(A) U2OS cells were transfected with the indicated siRNAs and fixed 72 h later. For DNA content analysis, cells were stained with propidium iodide and analyzed by flow cytometry. Percentage of cells with a 4n DNA content is indicated. This is a representative example of three biological replicates. (B) Same as in A, this time cells were double stained using propidium iodide and Phospho-H3S10 antibody. Percentage of mitotic cells is indicated.