Relative gene expression of cytokines associated with anti-viral and T_H1 responses.

<p>Relative gene expression of the cytokines IL-12 and IFN-α in chicken thrombocytes at 1, 3, 8 and 18 hours post-treatment with low (1 µg/mL) and high (5 µg/mL) doses of the TLR ligands LPS and CpG ODN. Data represent mean expression levels of target genes in 5 biological replicates relative to the house keeping gene β-actin ± standard error. Statistical significance between treatment groups and the medium control group was calculated using a paired student’s t test and was considered statistically significant if p≤0.05 (*) and p≤0.01 (**), and in the case of CpG ODN, considered statistically significant from the non-CpG ODN control group if p≤0.05 (#).