Quantification of honokiol-inhibited STAT-3 phosphorylation in gastric cancer cells and HUVECs.

<p>AGS (A) and MKN45 (B) quiescent human gastric cancer cells and HUVECs (C) were treated with and without honokiol (HK, 5–40 µM) for the indicated times. Whole cell lysates were prepared and analyzed by Western blotting for detection of phosphorylated STAT-3 (pSTAT-3, Tyr705). The blottings were reprobed with anti-STAT-3 antibodies for normalization. All data are presented as mean ± SEM of five independent experiments. *p<0.05 as compared with control.