GABAergic signaling at the Golgi cell – granule cell synapse is transiently inhibited following high frequency stimulation. <i>A,</i>

<p>GABAergic IPSCs were evoked in granule cells by stimulating Golgi cell axons at 0.1 Hz. High frequency stimulation (HFS, 10 pulses at 100 Hz) reduced both eIPSCs and I_tonic current. Depicted responses are averages of 5 to 10 eIPSCs, before (c1, c2) and after HFS, at 5 min (t1), 20 min (t2), and 40 min (t3). Inset shows superimposed traces of c1 and t1 scaled to the same amplitude indicating that the kinetics of the response were not changed. <b><i>B,</i></b> Histograms show averaged data from 12 cells for eIPSCs (black) and 10 cells for I_tonic (red). <b><i>C,</i></b> Time course of the changes in eIPSC amplitude following HFS for a representative cell, and <b><i>D,</i></b> averaged responses from 12 cells. <b><i>E,</i></b> Time course of the changes in I_tonic for a single cell, and <b><i>F</i></b><b>,</b> averaged values for 10 cells.