<p>(A) Tail DNA genotyping of GSK3β-flox and Cre for selecting snGSK3β-KO and littermate WT mice. Lane 1, GSK3β-flox homozygote and ePet1-Cre (+) = GSK3β knockout (KO); Lane 2, GSK3β-flox homozygote and ePet1-Cre (-) = GSK3β wildtype (WT); Lane 3, GSK3β-flox heterozygote and ePet1-Cre (-); Lane 4, GSK3β-flox heterozygote and ePet1-Cre (+). Mice with genotypes in Lane 1 and Lane 2 were used for experiments. (B) Immunostain of TpH2-positive cells (red), GSK3β-containing cells (green), and GSK3β/TpH2 co-stained cells in the midbrain (4x magnification) and the dorsal raphe (DRN) (63x magnification), and immunostain of GSK3β-containing cells (green) in the cerebral cortex (4x magnification). (C) quantification of immunostained TpH2-positive ...
<p>(<b>A</b>) Schematic representation of targeting construct and Wild type, Targeted (Flox-Neo), Fl...
<p>(A) Schematic diagram showing the mouse crosses that lead to the required genotypes. (B) Immunobl...
<p>(A) Schematic of the KO allele, in which exon 5 was deleted by homologous recombination in ES cel...
<p>A. Top row: schematic of mouse GSK-3α locus. Middle row: region containing exons 1–3 are depicted...
<p>Lane 1, ERβ<sup>+/+</sup> mice; Lane 2, ERβ<sup>+/-</sup> mice; Lane 3, ERβ<sup>-/-</sup> mice.</...
<p>(A) PCR results using gDNA obtained from tail biopsies of progeny generated by crossing electropo...
<p>(A) Schematic representation of the strategy for generation of the AP-4 ε (<i>AP4E1</i>) KO mouse...
<div><p>Brain homogenates were treated with or without PK as indicated prior to SDS PAGE analysis.</...
<p>(A) PCR products were separated on 1.5% agarose gel and the expected band sizes for WT and mutant...
<p>Scheme depicts the region within the Eps15 wildtype (WT) locus that was altered to generate the k...
<p>(<b>A</b>) Western blots analyzing pGSK3β and total GSK3β levels in control (shpLKO.1) and Sirt2 ...
<p>A. Comparison of CD5 and TCRγδ surface levels on DN γδ thymocytes from WT γδTCR Tg, Lck<sup>+/−</...
Glycogen synthase kinase-3 (GSK3) is a constitutively active protein kinase in brain. Increasing evi...
The Twitcher (Twi) mouse is a neurological Krabbe disease (KD, or globoid cell leukodystrophy) spont...
<p>(A) Schematic representation of the four different transgenic lines used to generate double trans...
<p>(<b>A</b>) Schematic representation of targeting construct and Wild type, Targeted (Flox-Neo), Fl...
<p>(A) Schematic diagram showing the mouse crosses that lead to the required genotypes. (B) Immunobl...
<p>(A) Schematic of the KO allele, in which exon 5 was deleted by homologous recombination in ES cel...
<p>A. Top row: schematic of mouse GSK-3α locus. Middle row: region containing exons 1–3 are depicted...
<p>Lane 1, ERβ<sup>+/+</sup> mice; Lane 2, ERβ<sup>+/-</sup> mice; Lane 3, ERβ<sup>-/-</sup> mice.</...
<p>(A) PCR results using gDNA obtained from tail biopsies of progeny generated by crossing electropo...
<p>(A) Schematic representation of the strategy for generation of the AP-4 ε (<i>AP4E1</i>) KO mouse...
<div><p>Brain homogenates were treated with or without PK as indicated prior to SDS PAGE analysis.</...
<p>(A) PCR products were separated on 1.5% agarose gel and the expected band sizes for WT and mutant...
<p>Scheme depicts the region within the Eps15 wildtype (WT) locus that was altered to generate the k...
<p>(<b>A</b>) Western blots analyzing pGSK3β and total GSK3β levels in control (shpLKO.1) and Sirt2 ...
<p>A. Comparison of CD5 and TCRγδ surface levels on DN γδ thymocytes from WT γδTCR Tg, Lck<sup>+/−</...
Glycogen synthase kinase-3 (GSK3) is a constitutively active protein kinase in brain. Increasing evi...
The Twitcher (Twi) mouse is a neurological Krabbe disease (KD, or globoid cell leukodystrophy) spont...
<p>(A) Schematic representation of the four different transgenic lines used to generate double trans...
<p>(<b>A</b>) Schematic representation of targeting construct and Wild type, Targeted (Flox-Neo), Fl...
<p>(A) Schematic diagram showing the mouse crosses that lead to the required genotypes. (B) Immunobl...
<p>(A) Schematic of the KO allele, in which exon 5 was deleted by homologous recombination in ES cel...