Cigarette smoke extract increases GM-CSF expression in B6Tert-1 cells.

<p>(A) Bar graph of real-time RT-qPCR data of GM-CSF mRNA expression in B6Tert-1 cells treated with 10% CSE in growth medium for 2 days. The relative GM-CSF mRNA expression level was determined against the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA level. Data are mean ± SEM. The asterisk (*) indicates a statistically significant difference (<i>p</i><0.05), when compared with the control (untreated) cells. (B) Bar graph of GM-CSF ELISA data of the secreted GM-CSF in B6Tert-1 conditioned medium. The medium was collected after 2 days of exposure to 10% CSE. The asterisk (*) indicates a statistically significant difference (<i>p</i><0.05), when compared with the control (untreated) cells. Data are mean ± SEM.