Both cell migration and invasion decrease with decreasing SP1 in SNU484 diffuse-type gastric carcinoma cells, and transcription of genes related to cell proliferation or migration was changed by <i>SP1</i> siRNA transfection in MKN28.

<p>A) SNU484 cells were transfected with <i>SP1</i> siRNA or control siRNA. Total mRNA was extracted to determine the expression of endogenous <i>VEGF</i> and <i>CDH1</i> by qRT-PCR. <i>HPRT</i> was used as a control. **, <i>P</i><0.01. B) Western blot data demonstrate that protein levels of VEGF were not changed by SP1 knockdown in SNU484. ACTB was used as a control. C) Cell proliferation, migration, and invasion were assessed after transfection with <i>SP1</i> or control siRNA in SNU484. **, <i>P</i><0.01. D) qRT-PCR of <i>SP1</i> along with genes involved in negatively regulating either cell proliferation or cell migration after the transfection of <i>SP1</i> siRNA in MKN28 cells demonstrates that SP1 knockdown promotes the elevation of the cell proliferation-inhibiting genes <i>CXCL10</i>, <i>ACVRL1</i> and <i>CDKN2D</i> and the reduction of the cell migration inhibition genes <i>BCL2</i> and <i>TRIB1</i> at the transcript level. <i>HPRT</i> was used as a control. *, <i>P</i><0.05; **, <i>P</i><0.01.