Functional characterization by fluorescence signal.

<p>(<b>A</b>) Col-GFP cells were grown to different cell densities and the fluorescence signal measured in an automated fluorescence reader. Following experiments were performed after plating cells at a density of 1.5×10³/well. (<b>B, C</b>) Cells were stimulated with indicated concentrations of CXCL9 for 24 hrs and the GFP content relative to untreated cells (<b>D</b>) and the cell number (<b>E</b>) was measured. (<b>D, E</b>) Cells were stimulated with indicated concentrations of ACC for 24 hrs and the GFP content relative to untreated cells (<b>D</b>) and the cell number (<b>E</b>) was measured. (<b>F</b>) Shows stimulation with indicated concentrations of PDGF-BB for 24 hrs, GFP content relative to untreated cells was measured. The increase of GFP reflects an increased cell number (not shown). Results represent three independent experiments, each experiment was done in 16 replicates, and error bars represent SEM.