Suppression of PRDX2 mRNA and protein expression in moDCs by means of lipid-mediated siRNA transfection leads to enhanced sensitivity of moDCs to tert-BHP.

<p><b>A.</b> Reduced PRDX2 mRNA levels in unstimulated moDCs after transfection with anti-PRDX2 siRNA [qPCR; 2^−ΔΔCt normalization using ACTB as reference gene and the non-transfected sample (---) as control (set to 1, n = 4)]. <b>B.</b> Reduction of PRDX2 protein in unstimulated moDCs monitored by Western Blot. Data were normalized using ACTB as reference gene (n = 4). <b>C.</b> CM-DCF fluorescence was used as index for intracellular ROS levels in non-transfected moDCs and moDCs transfected with PRDX2-targeting siRNA, with or without tert-BHP stimulation (200 μM, 30 min, n = 8). <b>D.</b> Neutral Red assay was used as index viability index of non-transfected moDCs and moDCs transfected with PRDX2-targeting siRNA, with or without tert-BHP stimulation (200 μM, 30 min, n = 8). **P<0.01 vs. untransfected (--) and negative control (neg. ctr), ^xxxP<0.001 vs. Unstimulated, paired Student's t-test (two-tailed).