Soaking <i>B. malayi</i> adult female worms in siRNA results in Bm-<i>tpp</i> gene-specific inhibition of expression.

<p>Quantative Real time PCR. Two female worms at different time interval (12, 24, 36, 48, 60 h) and 2 day after treatment were removed and analyzed for difference in <i>tpp</i> and <i>gpd</i> gene-specific transcript levels by qRT-PCR. The relative amounts of <i>tpp</i> and gpd amplicon were determined by using the comparative ΔCT method and normalizing against the endogenous control gene (Bm-tub-1). The median value of the control group was set to 100% and the percent relative mRNA level was shown. (A) Soaking <i>B. malayi</i> adult female worms in siRNA results in <i>tpp</i> gene-specific inhibition of expression. (B) siRNA treatment of adult worms non significantly effects the expression level of gpd mRNA. The criterion for statistical significance between the results was <i>p</i> value<0.05 was considered significant marked as *, <0.01 as highly significant**, <0.001 *** as very highly significant.