<i>In vitro</i> treatment of infective larvae with Bm-tpp siRNA results in to reduction in tpp gene expression and impairs their <i>in vivo</i> development in jirds.

<p>(A) siRNA treatment of infective larvae (L3) resulted in to significant loss of Bm-<i>tpp</i> transcript level after 24 and 48 h of incubation. (B) <i>In vitro</i> silencing of tpp gene in L3 reduced <i>in vivo</i> establishment of adult worm in jirds. L3 were cultured with <i>tpp</i> siRNA, negative siRNA and in medium for 24 h. After incubation ∼100 L3 were inoculated in the peritoneal cavity of jirds and animals were euthanized on 120 day post inoculation for recovery of adult worms. (C) Significant number of female worms recovered from siRNA treated group displayed defective embryogenesis. All female were recovered from animals were teased in PBS on glass slide. Condition of intrauterine contents (eggs, embryonic stages pre-Mf, Mf) was analyzed microscopically (Nikon). Percent of sterile females was calculated with respect to total female worm recovered and analysed. The criterion for statistical significance between the results was <i>p</i> value<0.05 was considered significant marked as *, <0.01 as highly significant**, <0.001 *** as very highly significant.