ELISA assay from fractionated conditioned media (CM) from 7PA2 cells and control CHO cells detected Aβ oligomers in the conditioned medium of 7PA2 cells but not of CHO control cells (a).

<p>Hippocampal slices were incubated with CM from fractions 12, 24 and 36 of control as well as 7PA2 cells. fEPSPs were recorded in response to Schaffer collateral commissural pathway stimulation at a range of stimulus from 10 V through to 60 V (b and d). Incubation with cell-derived Aβ, increased the response to stimulation at the CA1 synapse (<b>b</b>). compared to slices treated with conditioned media from the control CHO cells (<b>d</b>). The results are expressed as a percentage of the maximum fEPSP slope from control slices. Graphs showing representative results at 50 V in slices exposed to 7 PA conditioned medium (<b>c</b>) and to control CHO conditioned medium (<b>e</b>) show that cell derived Aβ increased fEPSP (student’s t-test p = 0.003 n = 5), whereas the control medium had no affect at 50 V (n = 5) (e).