Annexin V-PE labeling and mitochondrial transmembrane potential following exemestane treatment.

<p>(A) Annexin V-PE labelling of MCF-7aro cells treated with exemestane (15 µM) for 6 and 9 days of treatment. Cells cultured with T were considered as control and for 6 days present 3.54% and for 9 days 5.58% of Annexin V binding. Data presented in histograms were analysed with FlowJo Software (Tree Star, Inc), correspond to cells gated for negative 7-AAD staining and are representative of one independent assay. The grey filled line corresponds to control and the black line to exemestane at 15 µM. (B) Mitochondrial transmembrane potential (ΔΨm) of MCF-7aro cells treated with exemestane (15 µM) with or without 3-MA (1 mM), for 3 and 6 days. Viable cells and cells with ΔΨm loss were identified. Data presented in histograms were analysed with FlowJo Software (Tree Star, Inc) and are representative of one independent assay.