Effect of concentration of Fe(II) on non-enzymatic oxidation of Fe(II) and incorporation of the Fe(III) product into transferrin.

<p>Progress curves were recorded at 460 nm for formation of diferric-transferrin and the initial rates were plotted versus concentration of Fe(II). The error bars represent standard deviation of three independent measurements. Concentration of apo-transferrin was 100 µM. Buffer was 100 mM Hepes, 100 mM NaCl, pH 7.2. Measurements were performed at 37°C.