Subcellular distribution of DHBV DNA replication intermediates in HepG2 cells.

<p>(A) DSL212 cells were cultured in the absence of tetracycline for 6 days. Cytoplasm and nuclei were fractionated with QIAgen Qproteome Cell Compartment Kit by following the manufacturer's directions. DHBV core-associated DNA and Hirt DNA were extracted from whole cell, cytoplasm and nuclear fractions were analyzed by Southern blot assay. (B) DHBV core-associated DNA and Hirt DNA were extracted from whole cell, cytoplasm and nuclear fractions of HepG3 cells (a HepG2-derived stable cell line containing an integrated DHBV head-to-tail unit-length DNA dimer) were analyzed by Southern blot assay.