Phenotypic features of TCAL mice.

<p>(<b>A</b>) Radiography of an adult (12 week old) TCAL male mouse revealed widespread subcutaneous soft tissue opacities (white arrows); two magnified areas (a and b) are shown to illustrate detailed views. (<b>B</b>) Von Kossa stained sections from TCAL male mouse (36 week old) showing ectopic calcification in skin (arrow indicates mineralised panniculus fat), cutaneous striated muscle, heart, aorta, kidney and tongue submucosa. (<b>C</b>) Analysis of testes. Calcification was observed in the testicular arteries (left panel, von Kossa staining), and H&E sections (middle and right panels) of wild type (WT) and TCAL testis revealed disorganised seminiferous tubules and loss of spermatozoa in TCAL mice. (<b>D</b> and <b>E</b>) TUNEL staining of WT and TCAL testis and kidney sections, respectively, revealed increased apoptosis (green staining) in the tissues of TCAL mice.