RGV 50L silencing assay by siRNA.

<p>EPC cells, cultured in 24-well plates at about 8.0×10⁵ cells/ml, were transfected with siRNAs targeted to <i>50L</i> (siRNA-319, 594 and 811) and a negative control (siRNA-NC) at a final concentration of 150 nM, respectively. Then, 5 h later, the transfected and un-transfected cells were incubated with approximately 1 MOI RGV for 1 h and harvested at 24 h p.i. The silence effect of siRNAs on the expression of 50L was detected by western blot analysis. β-actin gene was used as an internal control.