Synapse-related gene synaptophysin in the dentate gyrus of adult ischemic rats in response to chronic mild stress and the effects of gamma-secretase inhibitor.

<p>Western blotting analysis using anti-synaptophysin was performed to investigate protein levels of synaptophysin (A) following chronic mild stress and the effects of gamma-secretase inhibitor DAPT on Day 28 after ischemia surgery. Real-time PCR was also performed to quantify gene expression of synaptophysin (B) in response to chronic mild stress and the effects of gamma-secretase inhibitor DAPT on Day 28 after ischemia surgery, number of each group = 6. Representative photographs from three to four independent experiments are shown. Statistically significant differences CMS, MCAO and MCAO+CMS compared with CON are indicated (^#<i>P</i><0.05), MCAO+CMS compared with MCAO are indicated (^▴<i>P</i><0.05), or MCAO+CMS+D compared with MCAO+CMS are indicated (^★<i>P</i><0.05), as determined by Bonferroni correction. CON, Control; CMS, Chronic mild stress; MCAO, Middle cerebral artery occlusion; D, DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenyl-glycine t-butyl ester).