MALDI-TOF mass spectra of HNP-1 reaction with CTA.
- Marta Castagnini (312271)
- Monica Picchianti (312272)
- Eleonora Talluri (312273)
- Massimiliano Biagini (247421)
- Mariangela Del Vecchio (312274)
- Paolo Di Procolo (312277)
- Nathalie Norais (159651)
- Vincenzo Nardi-Dei (312280)
- Enrico Balducci (312282)
Publication date
February 2013
DOIAbstract
<p>Mass spectra analysis confirmed the mono-ADP-ribosylation of HNP-1 by CTA after incubation at 30°C for 1h in the presence of 2 mM NAD. Upper panels (left side: spectrum of <i>m/z</i> 2500 – 5300, right side: zoomed spectrum of <i>m/z</i> 3800 – 4300) show the mass of the control reaction, i.e. HNP-1 incubated only with NAD without toxin (<i>m/z</i> 3442.12). Lower panels (left side: spectrum of <i>m/z</i> 2500 – 5300, right side: zoomed spectrum of <i>m/z</i> 3800–4300) represent the unmodified HNP-1 peptide and the product of ADP-ribosylation peptide by CTA (<i>m/z</i> 3983.15). Stars (*) correspond to Sinapinic Acid adducts (+206 Da).</p
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