Effect of DMOG pretreatment on expanding survival area of ischemic skin flaps in mice.

<p>A, BALB/c mice were randomly assigned to the control group or the experimental group with intraperitoneal DMOG pretreatment (400 mg/kg body weight), followed by evaluation of flap survival on postoperative day 7. B, Design of ischemic random pattern skin flap and vascular distribution on the mouse dorsum. The flap was designed not to include any major pedicles arising from the deep circumflex iliac vessels and lateral thoracic vessels (white arrows). The distal parts of the flaps exhibited tissue necrosis due to disruption of the blood supply. C, Percentages of the survival area of the flap and dividing by the total area of the flap. Representative ischemic flaps show grossly better flap survival in the DMOG group. D, Effects of timing of DMOG pretreatment on the expansion of flap survival. DMOG pretreatment was performed 2 days or 1 day before the surgery or on the day of the surgery. E, Effect of dose of DMOG pretreatment on the expansion of flap survival. DMOG pretreatment was performed 2 days prior to surgery. The percentages of flap survival ± SEM were measured on postoperative day 7. *<i>P</i>>0.05; ***<i>P</i>>0.001.