Microfluidic Chip housing three amplification chambers.

<p>(A) An exploded view of the chip used for genotyping of malaria-transmitting mosquitoes. The chip consists of three layers: a top PMMA film; a PMMA chip body, and a PCR Sealers™ tape bottom. The various features of the chip body were milled with a CNC machine. (B) Cross-sectional view of the assembled, inverted chip loaded with a FTA disc. In the test chambers, the disks carry pieces of a mosquito leg. (C) A photograph of the chip.